[AF] urgente : identif icación

Jue Mayo 12 20:45:52 CEST 2005

Ademas de lo ya dicho como test de cloracion, es un agente cancerigeno y 
tambien se ha utilizado para detectar la posible aparicion de sangre en 
distintas muestras fisiologicas, siguiendo la hemoglobina.

Espero que resulte de utilidad

Eduardo L. Mariño

Incluyo bibliografia:

1) J Coll Physicians Surg Pak. 2004 Mar;14(3):157-60. Related Articles, Links  
Qualitative assessment of bacteriological quality and chlorination status of 
drinking water in Lahore.
Anwar MS, Chaudhry NA, Tayyib M.

Department of Pathology, Postgraduate Medical Institute, Lahore. 
amir1985pk en hotmail.com

OBJECTIVE: Qualitative assessment of bacteriological quality and chlorination 
status of drinking water in Lahore. DESIGN: Descriptive study. PLACE AND 
DURATION OF STUDY: Lahore city from October 2000 to September 2001. MATERIALS 
AND METHODS: A total of 2160 water samples from distribution system were 
tested from nine different localities of Lahore. These localities represented 
areas with different socioeconomic conditions (SEC). Twenty water samples were 
tested from each locality from the same taps each month. All the water samples 
were subjected to H2S strip test for determination of bacteriological 
contamination as well as orthotolidine test for detection of chlorine. 
RESULTS: Four hundred and forty-six (20.64%) samples were positive for 
bacterial contamination. It was observed that contamination was the maximum in 
low SEC areas (32.22%), followed by intermediate SEC areas (18.47%) and high 
SEC areas (11.25%). The difference was found to be statistically significant 
(p<0.01) among different areas. Only 27.73% samples were chlorinated. 
Positivity of samples for chlorine was the lowest in areas with low SEC
(20.69%) and highest (32.77%) in areas with high SEC, the difference being 
statistically significant. Maximum contamination was present in samples tested 
during summer months(June-August) of the year (31.11%), followed by autumn 
months (September-November) of the year (20.9%), spring months (March-May) of 
the year (18.7%) and winter months (December-February) of the year (11.85%). 
Samples tested during summer months showed the highest positivity (77.96%) for 
chlorine (p<0.001) as compared with other months of year. Among chlorinated 
samples 12.32% showed bacterial contamination. However, contamination was 
significantly higher (p<0.001) among non-chlorinated samples. CONCLUSION: 
Bacterial contamination of piped water is a significant problem in Lahore. 
Regular chlorination and monitoring of water supplies can improve it.

2)Aliment Pharmacol Ther. 1996 Jun;10(3):333-7. Related Articles, Links  
How accurate is the determination of blood in gastric juice? Comparison of 
peroxidase and porphyrin methods.
Woods KL, Kiefe C, Graham DY.

Department of Medicine, VA Medical Center, Houston, Texas, USA.

BACKGROUND: Mucosal bleeding is frequently used as a measure of gastric 
mucosal integrity. We compared the orthotolidine method for quantifying 
haemoglobin in gastric juice with a method based on measurement of fluorescent 
porphyrins extracted from haem. We also investigated whether acid or pepsin 
had a deleterious effect on the results of either method. METHODS: We compared 
the effects of pH (2 or 7), time (up to 180 min), haemoglobin concentration 
and the addition of pepsin (2.5, 5 or 7.5 kU/mL hog pepsin) on the accuracy of 
the two methods. RESULTS: With the orthotolidine method there was a time-
dependent decline in detectable haemoglobin concentration (P < 0.02) at pH 2 
that was not seen at pH 7. The time- and pH-dependent decline in detectable 
haemoglobin was not seen using the porphyrin assay. CONCLUSION: The widely 
used orthotolidine method for determination of blood in gastric juice is less 
reliable than the porphyrin method as it is more likely to be influenced by 
acidic conditions in the stomach.

3)Digestion. 1976;14(5-6):409-14. Related Articles, Links  
A sensitive method for measuring haemoglobin in gastric contents.
Fisher MA, Hunt JN.

A sensitive method for measuring haemoglobin in gastric contents using the 
rate of change of optical density of a mixture of orthotolidine and hydrogen 
peroxide in the presence of haemoglobin, is described. The method can measure 
the blood normally lost in 10 min from the gastric mucosa.

PMID: 10217 [PubMed - indexed for MEDLINE] 

Missatge de Santiago Ricarte <sricarte001 en cofb.net>:

> Un cliente me acaba de traen un envase lleno de liquido que se ha encontrado
> en el portal de su casa.
> Es  Otolidina.
> He visto que esta relacionado con algo del agua y la cloración. y es toxico
> Antes de llamar a la guardia urbana.
> Me podeis dar mas pistas y usos.

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